| Abstract: | The effect of influenza C virus, strain JJ/50, on the development of chicken embryos infected at 10 or 12 days was documented by microscopic techniques, as well as by gross observations of embryos or chicks at hatching. The infected, newly hatched chicks displayed marked abnormalities in their feathering. Such abnormalities were observed neither in mock-infected embryos nor in embryos injected with virus which had been previously treated with specific influenza C virus antibody. At a microscopic level, the abnormalities apparently are a result of hypertrophy and/or hyperplasia of the developing barb and barbule cells. Further, the additional development of integumental necrotic foci was correlated with the development of relatively high viral titers (greater than 256) as measured by hemagglutination (HA). Embryos infected after 12 instead of 10 days incubation showed normal feathering at hatching. Infection at 12 days, however, was correlated with the development of relatively low viral titers (HA = 4) and limited degeneration of the respiratory epithelium. The relationship of teratogenic effects to the site of viral replication in rapidly differentiating tissue is discussed. |
