Proteasomal activity modulates TGF-ss signaling in a gene-specific manner |
| |
Authors: | Zhang Fan Mönkkönen Mia Roth Stina Laiho Marikki |
| |
Affiliation: | Department of Physiology and Biophysics, Case Western Reserve School of Medicine, Cleveland, OH 44106-4970, USA. |
| |
Abstract: | Myosin heavy chain kinase A (MHCK A) modulates myosin II filament assembly in the amoeba Dictyostelium discoideum. MHCK A localization in vivo is dynamically regulated during chemotaxis, phagocytosis, and other polarized cell motility events, with preferential recruitment into anterior filamentous actin (F-actin)-rich structures. The current work reveals that an amino-terminal segment of MHCK A, previously identified as forming a coiled-coil, mediates anterior localization. MHCK A co-sediments with F-actin, and deletion of the amino-terminal domain eliminated actin binding. These results indicate that the anterior localization of MHCK A is mediated via direct binding to F-actin, and reveal the presence of an actin-binding function not previously detected by primary sequence evaluation of the coiled-coil domain. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|