三七愈伤组织的培养

在MS培养基中加入不同浓度的KT和2,4-D,综合考虑三七愈伤组织生长缓慢兼顾皂甙含量,较合适的KT浓度为0.7ppm,较合适的2,4-D浓度在2—3ppm之间。在培养基中补充各种添加剂,结果以椰子乳和水解乳蛋白较好。综合生长和皂甙含量以20％的椰子乳和0.7％的水解乳蛋白较合适。从21个三七愈伤组织无性繁殖系中筛选山了5个较优的无性系,特别是其中04号无性系更优,无论生长速率还是总皂甙含量都更高。通过以上研究,使三七愈伤组织的生长速率达220毫克/升/天,是原初培养愈伤组织(54.0mg干重/升/天)的4倍。愈伤组织中总皂甙含量高达13％,是原初培养愈伤组织(5.37％)的2.4倍,为原植物的3倍。从而证明了三七培养组织次级代谢的全能性是可调节的,为三七细胞工程的工业生产应用打下了初步基础。

CALLUS CULTURE FROM PANAX NOTOGINSENG

The growth rate was 54.0 mg D.W./I/day and saponin content was 5.37% for original culture callus from Panax notoginseng. As we considered low growth rate of P. notoginseng callus and saponin content, the suitable KT concentration and 2,4-D concentration should be 0.7 ppm and 2.5 ppm respectively when the different concentration of KT and 2,4-D were added in to medium. Coconut milk (CM) and lactalbumin hydrolysatc (LH) had more efficent nutrient regulation in the callus culture. 20% CM and 0.7% LH were therefore more suitable both growth and saponin content respectively. Five fine clones had been selected from twenty-one clones of P. notoginseng callus, amony which No. 04 which growth rate was as high as 219.2 mg D.W./I/day and that saponin content 12.8% was finest. The growth rate of the callus was reached as high as 220 mg D.W./I/day through the studies on metabolism regulation in callus culture of P. notoqinseng and this was four times as that of the original culture callus. The total saponin content in the callus was 13% which was 2.4 times as that the original culture callus and as three times of the original plant. The results confirmed that the totipotency of secondary metabolism in culture cell of P. notoginseng might be regulated.