A fast, simple, and reliable high-yielding method for DNA extraction from different plant species |
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Authors: | Raul Tapia-Tussell Andres Quijano-Ramayo Rafael Rojas-Herrera Alfonso Larque-Saavedra Daisy Perez-Brito |
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Affiliation: | Laboratorio GeMBio, Centro de Investigación Científica de Yucatán, Calle 43, #130, Col. Chuburná de Hidalgo, Mérida 97200, Yucatán, México. |
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Abstract: | Genetic studies and pathogen detection in plants using molecular methods require the isolation of DNA from a large number of samples in a short time span. A rapid and versatile protocol for extracting high-quality DNA from different plant species is described. This method yields from 1 to 2 mg of DNA per gram of tissue. The absorbance ratios (A260/A280) obtained ranged from 1.6 to 2.0. A minimal presence of contaminating metabolites (as polymerase chain reaction [PCR] inhibitors) in samples and a considerable savings in reagents are characteristics of this protocol, as well as the low cost of the analysis per sample. The quality of the DNA was suitable for PCR amplification. |
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Keywords: | Agavaceae Cariacaceae Cucurbitaceae DNA isolation Graminaeae Palmae PCR Rutaceae Solanaceae SSR |
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