应用RAPD技术辅助蝴蝶兰辐射育种

应用RAPD技术对蝴蝶兰辐射诱变苗进行分析。结果表明,对蝴蝶兰5种不同形态的诱变苗和对照苗进行PCR扩增,13个引物所产生的RAPD谱带大小为200~3 000 bp,共扩增77条带,多态性带61条,多态率79.22%。13个引物平均扩增的带数为5.92,引物S0036最多,为9条带。比较诱变苗与对照苗谱带,两者有差异,表明诱变苗是由基因突变引起。

Radioactive Breeding of Phalaenopsis spp. Using RAPD Method

Using RAPD method,radiation-induced mutant seedlings of Phalaenopsis were studied.Five mutant seedlings of different formation and the control were amplified by PCR method,13 arbitrary primers amplified 77 bands ranging from 200bp to 3000bp with an average of 5.92 bands per primer,among them 61 bands were polymerbhic and the percentage was 79.22%.The most bands were obtained from primer S0036.Compared the bands of mutant seedlings with those of control,there were differences among them and the results indicated that mutant seedlings were caused by gene mutation.