Transposable elements ofXanthomonas campestris pv.citri originating from indigenous plasmids |
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| Authors: | Jenn Tu Huei-Rong Wang Shau-Feng Chang Yuh-Chyang Charng Rudi Lurz Beate Dobrinski and Wen-Chuan Wu |
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| Institution: | (1) Institute of Botany, Academia Sinica, Taipei, Taiwan 11529, ROC;(2) Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 63-73, D-1000 Berlin 33;(3) Graduate Institute of Plant Pathology, National Chung Hsing University, Taichung, Taiwan 40227, ROC |
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| Abstract: | Summary After introduction of the broad host range plasmid RP4 inXanthomonas campestris pv.citri strain XAS4501 twoXanthomonas transposable elements, ISXC4 and ISXC5, were isolated. These elements were found to be capable of transposition inEscherichia coli. Restriction analysis, DNA hybridization and heteroduplex experiments revealed that ISXC4 and ISXC5 are about 5.55 and 6.95 kb in size, respectively, possess inverted repeats about 50±18 bp in length and share DNA homology
in their left (5.0 kb) and right (0.6 kb) ends. ISXC4 and ISXC5 were found to originate from plasmids pXW45N and pXW45J, which are indigenous replicons inX. campestris pv.citri strain XW45. |
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| Keywords: | Xanthomonas campestris pv citri Transposable elements Indigenous plasmids |
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