Transfer of arachidonic acid between phospholipids in rat liver microsomes |
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Authors: | R F Irvine R M Dawson |
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Institution: | Biochemistry Department, ARC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT UK |
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Abstract: | Phosphatidylcholine and phosphatidylinositol labelled with radioactive oleic, arachidonic or linoleic acids in the 2-acyl position were prepared. Rat liver microsomes were incubated with either lysophosphatidylcholine or lyso-phosphatidylinositol and the opposite 2-acyl-labelled phospholipid, and were found to catalyse a transfer of fatty acids between the two phospholipids. This was shown to be a direct Co-enzyme A-mediated transfer that does not involve a free fatty acid intermediate (i.e. it is independent of phospholipase A2 activity). Arachidonoyl transfer took place at about four times the rate of linoleoyl transfer; oleoyl transfer was not detectable. The role of direct arachidonoyl transfer to phosphatidylinositol in the controlled release of arachidonic acid for prostaglandin synthesis is discussed. |
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