肺癌转移相关蛋白的比较蛋白质组分析与鉴定

采用比较蛋白质组技术对肺巨细胞癌高、低转移株的蛋白质表达谱进行双向电泳分离和MALDI-TOF分析，并在蛋白质和mRNA水平进行进一步验证，成功鉴定了11个肺癌转移相关蛋白.其中，候选蛋白膜联蛋白1(ANX1)、细胞骨架蛋白(CK18)、Rho-GDP解离抑制剂1(GDIR)、原肌球蛋白3(TPMF)、蛋白谷氨酰胺γ-谷氨酰转移酶(TGLC)和白介素18(IL-18)在肺巨细胞癌高转移株显著高表达，而候选蛋白核氯离子通道蛋白1(CLI1)、蛋白质二硫键异构酶(ER60)、肌酸激酶、硫氧还蛋白过氧化物酶1(PDX1)和热休克蛋白60(CH60)在肺巨细胞癌高转移株显著低表达.大多数的候选蛋白可通过调控肿瘤细胞的生长、迁移、粘附、凋亡和肿瘤免疫等环节来影响肿瘤细胞的侵袭和转移能力.迄今为止，还未见候选蛋白CLI1和IL-18与肺癌转移相关的报道，提示这两种蛋白质可能为新的肺癌转移相关蛋白.

Characterization and Identification of Metastasis-associated Proteins of Lung Cancer by Comparative Proteome Analysis

Comparative proteomic analysis, combined with 2-DE separation and MALDI-TOF identification, was utilized to compare the protein expression profiles between highly and lowly metastatic subpopulations(i.e.PLA801D and PLA801C) and 11 metastasis-associated proteins were identified and further validated by 1-D Western blotting, Northern blot and/or semi-quantitative RT-PCR analysis. Compared with that in lowly metastatic subpopulation, CK18, TGLC, GDIR, TPMF, IL-18 and ANX1 were significantly up-regulated, while ER60, CH60, PDX1, CLI1 and KCRB were significantly down-regulated in highly metatsatic subpopulation. Most of the candidate proteins have been evidenced to be somehow associated with various aspects of tumor metastasis such as cell growth, motility, invasion, adhesion, apoptosis and tumor immunity, etc. Up to now, CLI1 and IL-18 have not been reported to be associated with lung metastasis, which implied that CLI1 and IL-18 might be new metastasis-associated proteins of lung cancer.