Molecular characterization and crystallization of Diocleinae lectins |
| |
Authors: | Calvete J J Thole H H Raida M Urbanke C Romero A Grangeiro T B Ramos M V Almeida da Rocha I M Guimarães F N Cavada B S |
| |
Institution: | Instituto de Biomedicina, C.S.I.C., Jaume Roig 11, 46010, Valencia, Spain. |
| |
Abstract: | Molecular characterization of seven Diocleinae lectins was assessed by sequence analysis, determination of molecular masses by mass spectrometry, and analytical ultracentrifugation equilibrium sedimentation. The lectins show distinct pH-dependent dimer-tetramer equilibria, which we hypothesize are due to small primary structure differences at key positions. Lectins from Dioclea guianensis, Dioclea virgata, and Cratylia floribunda seeds have been crystallized and preliminary X-ray diffraction analyses are reported. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|