Locust vitellogenin receptor: an acidic glycoprotein with N- and O-linked oligosaccharides |
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| Institution: | 1. College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao, China;2. Department of public course teaching, University of Health and Rehabilitation Sciences, Oingdao, China;3. Department of Physics, Pukyong National University, Busan 48513, South Korea;4. School of medicine and pharmacy, Ocean University of China, No. 5 Yushan road, Qingdao, China;5. Bassars college of future agricultural science and technology, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao, China;6. Department of Spine Surgery, Affiliated Hospital of Qingdao University, No. 16 Jiangsu road, Qingdao, China;7. Qingdao Shenkang Stem Cell Biotechnology Co., Ltd, Qingdao, China |
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| Abstract: | - 1.1. The locust vitellogenin (VTG) receptor which is embedded in oocyte plasma membranes is a glycoprotein.
- 2.2. With various lectins oligosaccharide units have been identified, among them neuraminic acid linked to Gal or GalNAc, mannose chains, Gal linked to GalNAc or GlcNAc and fucose linked to GlcNAc.
- 3.3. With specific enzymes it could be shown that mannose and most other oligosaccharides are O-linked while others like fucose are N-linked.
- 4.4. Enzymatic removal of all O-linked carbohydrates resulted in a drop of the molecular mass of the receptor protein from 200,000 to 110,000.
- 5.5. A total of N- and O-linked oligosaccharides of 54% was calculated.
- 6.6. The isoelectric point of the receptor was found to be at pH 3.4 increasing slightly after removal of neuraminic acid.
- 7.7. Removal of neuraminic acids destroyed the binding ability for VTG.
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