Ultrastructure of maturing fish (Oreochromis niloticus) and snake (Waglerophis merremii) erythroid cells with regard to hemoglobin biosynthesis |
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| Affiliation: | 1. Laboratory of Electron Microscopy, Brazil;2. Laboratory of Hematology, Institute Butantan, CEP 05504, Brazil;3. Faculdade de Medicina Veterinaria, Universidade de São Paulo, SP, Brazil;1. Unidad de Neurobiología Dinámica, Department of Neurochemistry, Instituto Nacional de Neurología y Neurocirugía, Mexico City, Mexico;2. Lab. BL-305, Instituto de Fisiología Celular, Department of Cognitive Neuroscience, Universidad Nacional Autónoma de México, Mexico City, Mexico;1. Nano-Biotechnology Laboratory, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium;2. Department of Chemistry and Forensics, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK;3. Department of Environmental Sciences, Jozef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia;1. Key Laboratory of Metallurgical Equipment and Control Technology of Ministry of Education, Wuhan University of Science and Technology, China;2. Hubei Key Laboratory of Mechanical Transmission and Manufacturing Engineering, Wuhan University of Science and Technology, China;1. School of Energy and Power Engineering, Beihang University, Beijing 100191, PR China;2. National Key Laboratory of Science and Technology Aero-Engine Aero-thermodynamics, Beihang University, Beijing 100083, PR China;3. Xi''an Modern Control Technology Research Institute, Xi''an 710054, PR China;4. School of Astronautics, Beihang University, Beijing 100191, PR China;5. Beijing Advanced Innovation Center for Big Date-based Precision Medicine, Beihang University, Beijing 100083, PR China |
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| Abstract: | - 1.1. Fish and snake immature erythrocytes were submitted to a comparative ultrastructural study, analysing changes in organelles involved in hemoglobin (Hb) biosynthesis.
- 2.2. Iron uptake occurs probably via transferrin, and ferruginous compounds accumulate as siderosomes, taken as iron sources for heme biosynthesis, later on caught by a double lamella.
- 3.3. Mitochondrial membrane of the inner camera differentiates to lamellated bodies that, sucessively, give rise to expansions for ferruginous material and globin chains captation, constituting prehemosomal vesicles, which become condensed vesicles, followed by prohemosomes.
- 4.4. Through an internal membrane rearrangement, prohemosomes change to hemosomes wherein, hypothetically, heme and the globin chains assembly may occur.
- 5.5. In both fish and snake erythroid cells, all stages for hemosomegenesis are similar to the stages found in erythroid cells of other vertebrate species, including humans, except that fish cells often present single organelles of still unknown function, void of internal membrane.
- 6.6. Through electrophoresis of the respective supernatants obtained after osmotical lysis of the organellar fractions, it was shown that fish hemosomes contain three Hb patterns, while snake hemosomes present two patterns.
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