Vitellogenin and vitellin of a millipede Spirostreptus asthenes: Occurrence,isolation and partial characterization |
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| Institution: | 1. Dipartimento di Biomedicina, Neuroscienze e Diagnostica avanzata (BIND), Università degli Studi di Palermo, Via del Vespro 129, 90127 Palermo, Italy;2. Dipartimento di Fisica Chimica – Emilio Segrè (DiFC), Università degli Studi di Palermo, Viale delle Scienze, Ed. 17, 90128 Palermo, Italy;3. CIRCMSB ? Consorzio Interuniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici, Via Celso Ulpiani, 27, 70125 Bari, Italy;4. Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Plesso di Biochimica, Via del Vespro 129, 90127 Palermo, Italy;1. Department of Plant Biotechnology, College of Agriculture, Vellayani, Post Box No. 1603, Ernakulam North P.O., Kochi, 682 018, India;2. Marine Biotechnology Division, ICAR- Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O., Kochi, 682 018, India;3. Fishery Environment Management Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O., Kochi, 682 018, India;1. Department of Foodservice Management and Nutrition, Sangmyung University, 7 Hongji-dong, Jongno-gu, Seoul 110-743, Republic of Korea;2. Division of Industrial Promotion Research, Korea Food Research Institute, 62 Anyangpangyo-ro 1201 beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea;3. Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Scienece, 7-45 Songdo-dong, Yeonsu-ku, Incheon 406-840, Republic of Korea;4. Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea;5. Department of Food Science and Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea;1. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan;2. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan;3. Department of Basic Biology in the School of Life Science, SOKENDAI, Okazaki, Aichi, 444-8585, Japan;1. Laboratory of Insect Genetics and Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan;2. College of Animal Sciences, Zhejiang University, China |
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| Abstract: | Vitellogenin (Vg), the yolk precursor protein and its product vitellin (Vn) have been identified in hemolymph and fat body of females and mature oocytes in the millipede Spirostreptus asthenes employing double immunodiffusion technique. These two proteins are absent in males indicating that they are female specific. Immunoelectrophoresis has shown that there is only one vitellogenic protein present in S. asthenes. Vg and Vn were isolated by gel filtration. Polyacrylamide gel electrophoresis (PAGE) analysis revealed that Vg and Vn are glycolipoproteins. Vg contains 48.8% protein, 2.2% carbohydrate and 48.9% lipid. Vn is comprised of 52% protein, 2.3% carbohydrate and 45.4% lipid. The lipid components of Vg and Vn include mainly phospholipids such as phosphatidic acid, sphingomyelin, phosphatidyl choline, phosphatidyl ethanolamine and cholesterol. On SDS-PAGE analysis both Vg and Vn yielded five sub units each. The molecular weight of the sub units of Vg was found to be 135, 115, 105, 73 and 56 kDa and those of Vn were 125, 110, 100, 68, and 53 kDa. The vitellogenic system of S. asthenes resembles that of insects. The phylogenetic relationship of the vitellogenic system of this millipede with other arthropod groups is discussed. |
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