Characterization of nuclear matrix proteins of Physarum polycephalum and mammalian cells |
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Affiliation: | 1. Department of Microbiology, University of Innsbruck-Medical School, Fritz-Preglstr. 3, A-6020 Innsbruck, Austria |
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Abstract: | Analysis of the nuclear matrix of Physarum polycephalum and renal epithelial cells in culture (LLC-PK1) reveals a complex protein pattern. Applying various experimental protocols we observe only negligible differences in the final nuclear matrix protein pattern, in Physarum as well as in LLC-PK1 cells. Immunoblotting with a variety of antibodies against intermediate filament proteins and with antinuclear autoantibodies demonstrates the presence of intermediate filament proteins as components of the nuclear matrix. Preparation of type I and type II matrix structures does not yield different protein compositions, neither in Physarum nor in differentiated LLC-PK1 cells; therefore in both systems a distinction between these two types of matrices is questionable. |
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