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The transparent accessory reproductive gland secretes a polypeptide into the hemolymph of male Rhodnius prolixus |
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| Institution: | 1. Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Cátedra de Morfología Animal, Instituto de Investigaciones Biológicas y Tecnológicas (IIByT-CONICET), Avda. Vélez Sarsfield 299, Córdoba X5000JJC, Argentina;2. Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Cátedra de Introducción a la Biología, Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT-CONICET), Avda. Vélez Sarsfield 299, Córdoba X5000JJC, Argentina;3. Laboratorio de Investigación en Triatominos (LIT), Centro de Referencia de Vectores (CeReVe), Ministerio de Salud de la Nación, Hospital Colonia, Pabellón Rawson calle s/n, Santa María de Punilla, Córdoba, Argentina;4. Universidad Nacional de Córdoba, Grupo de Ecología y Control de Vectores, Instituto de Diversidad y Ecología Animal (IDEA-CONICET), CONICET Cordoba, Rondeau 798 (Jardín Zoológico) AVP, Córdoba X5000, Argentina |
| Abstract: | The transparent accessory reproductive gland of Rhodnius prolixus synthesizes and accumulates a variety of polypeptides. Ouchterlony immunodiffusion demonstrates that the hemolymph contains proteins which react with polyclonal antibodies against extracts of transparent accessory glands. Accessory glands and hemolymph contain a 170 kDa polypeptide with similar mobility on SDS-polyacrylamide gel electrophoresis. This polypeptide reacts with antibodies against extracts of accessory glands. Surgical removal of the accessory glands prevents the appearance of the 170 kDa polypeptide in the hemolymph. In vivo labeling of accessory gland proteins with a mixture of 14C]amino acids demonstrates that the newly synthesized TARG polypeptide appears in the hemolymph between days 2 and 3 after feeding. It is concluded that a specific polypeptide which is synthesized in the transparent accessory gland is exported to the hemolymph. |
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