Incorporation of arylphorins (LSP-1) and LSP-2 like protein into the integument of Ceratitis capitata during pupariation |
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| Affiliation: | 1. Department of Chemistry, UAE University, P.O. Box 15551 Al-Ain, UAE;2. Berkeley Global Science Institute, University of California Berkeley, CA 94720, United States;1. Pharmaceutical Chemistry Department, Pharmacy College, Al-Nahrain University, Baghdad, Iraq;2. Department of Civil Engineering, Dijlah University College, Al-Masafi Street, Baghdad 00964, Iraq;3. Department of Medical Laboratory Techniques, Dijlah University College, Baghdad 10021, Iraq;4. Medical Laboratory Techniques Department, Al-Farahidi University, Baghdad, Iraq;5. Anesthesia Techniques Department, Al-Mustaqbal University College, Babylon, Iraq;6. Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq;7. Department of Optics Techniques, Dijlah University College, Al-Masafi Street, Al-Dora, Baghdad 00964, Iraq;8. College of Technical Engineering, The Islamic University, Najaf, Iraq;9. Medical Laboratory Techniques Department, Al-Turath University College, Baghdad, Iraq;10. Department of Chemistry, Govt. Nagarjuna PG College of Science, Raipur, 492010, India |
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| Abstract: | The arylphorins and LSP-2 like polypeptide were detected by immunoblotting analysis during development in the integument of C. capitata. In vitro translation of total RNA from fat body and integument during pupariation, clearly revealed that the polypeptides under consideration were exclusively synthesized in the fat body. Furthermore, in vitro experiments demonstrated that radiolabeled arylphorins and LSP-2 like polypeptide were taken up by the integument, in an undegraded state. Immunofluorescence experiments in cross sections of wandering stage larvae and white pupae revealed that the LSP-2 like polypeptide was mainly localized in the epidermal cells, and a very weak signal was also given by the cuticle. Furthermore, the presented results indicated that a small portion of the extracted proteins exist in high molecular weight aggregate(s). |
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