Biochemical and functional characterization of Rapana thomasiana hemocyanin |
| |
| Institution: | 1. Institute of Organic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;2. C.N.R. Centre for the Physiology and Biochemistry of Hemocyanins and other Metalloproteins, I-35131 Padova, Italy;3. Department of Biology, University of Padova, 35131 Padova, Italy (Tel: 049 828 6349) (Fax: 049 828 6374);4. Department of Organic Chemistry, University of Padova, 35131 Padova, Italy;1. Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Laboratory of Cerebrovascular Pharmacology, College of Pharmaceutical Science, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, Suzhou, Jiangsu 215123, China;2. Department of Anesthesiology and Perioperative Medicine, Suzhou Science and Technology Town Hospital, and Institute of Clinical Medicine, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215153, China;1. Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russia;2. Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, 660041, Russia;3. Krasnoyarsk State Agrarian University, 90 Mira Prospect, Krasnoyarsk, 660049, Russia;4. SB RAS Genomics Core Facility, Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, 630090, Russia;1. The Center for Genome Architecture, Baylor College of Medicine, Houston, TX 77030, USA;2. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA;3. Department of Computer Science, Department of Computational and Applied Mathematics, Rice University, Houston, TX 77005, USA;4. Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA;5. Department of Biology, MIT, Cambridge, MA 02139, USA;6. Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA;7. Center for Theoretical Biological Physics, Rice University, Houston, TX 77030, USA;1. Department of Soil, Plant and Microbial Sciences, Michigan State University, East Lansing, MI, USA;2. DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI, USA;3. Department of Soil Ecology, Helmholtz Centre for Environmental Research – UFZ, Halle (Saale), Germany |
| |
| Abstract: | - 1.1. The hemocyanin (Hc) of the marine gastropod mollusc Rapana thomasiana was collected from animals living on the west coast of the Black Sea and characterized for its biochemical and functional properties.
- 2.2. This Hc is very similar to other gastropod Hcs as far as amino acid composition, general structure and reactivity of the binuclear copper active site are concerned.
- 3.3. Some peculiarities in the dissociation-reassociation pattern are observed in comparison to other gastropod Hcs, in particular with respect to the ability to form sopramolecular aggregates.
- 4.4. Changes in pH disclose a strong reverse Bohr effect. Different R and T states are required to describe the oxygen binding curves at the different pHs.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
