Accelerated communication the direct contractile effect of gastrin releasing peptide on isolated gastric smooth muscle cells of the guinea pig |
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| Institution: | 1. Division of Cardiovascular Medicine, Central Arkansas for Veterans Healthcare System, Little Rock, AR;2. Division of Cardiovascular Medicine, University of Arkansas for Medical Sciences, Little Rock, AR;1. Department of Anaesthesia and Intensive Care, Oslo University Hospital, Oslo, Norway Gulleraasveien 30 B, 0779 Oslo, Norway;2. Department of Clinical Medicine, University of Oslo Gulleraasveien 30 B, 0779 Oslo, Norway;3. White Mountain Institute, 41299 Tallgrass, The Sea Ranch, CA 95497, USA |
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| Abstract: | Smooth muscle cells isolated from the gastric muscle layers of the guinea pig were used to determine whether gastrin releasing peptide (GRP) can cause contraction by exerting a direct action on muscle cells. In addition, the inhibitory effect of 8-( N,N-diethylamino )-octyl-3,4,5-trimethoxybenzoate hydrochloride ( TMB-8 ), an inhibitor of intracellular Ca2+ release, and verapamil, a Ca2+ channel blocker, on the GRP-induced contraction of gastric smooth muscle cells were examined. GRP elicited a contractile response of gastric muscle cells in a dose-dependent manner. The ED50 was 13 pM. TMB-8 significantly inhibited the contractile effect of GRP in gastric muscle cells. These results demonstrate the direct action of GRP on the gastric smooth muscle cells of the guinea pig, and the importance of Ca2+-release from intracellular calcium stores in the contractile response to GRP. |
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