The restriction analysis of nDNA from the corvids (Passeriformes,aves) |
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| Affiliation: | 1. Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China;2. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;3. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;4. College of Biological Science & Engineering, North Minzu University Yinchuan 750002, China;1. Key Laboratory of Grassland Resources of the Ministry of Education, College of Grassland, Resources, and Environment, Inner Mongolia Agricultural University, Hohhot, China;2. Center for Comprehensive Test and Demonstration, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, China;1. Departamento de Ciencias Ecológicas, Universidad de Chile, Las Palmeras, Las Palmeras 3425, Santiago, Chile;2. Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Av. Libertador Bernardo O’Higgins 3363, Santiago, Chile;3. Center of Applied Ecology and Sustainability (CAPES), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Libertador General Bernardo O′Higgins 340, Santiago, Chile |
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| Abstract: | - 1.1. The nDNA of carrion crow Corvus corone L., hooded crow C. cornix L., their hybrids, as well as magpie Pica pica L., were digested by the tetranucleotide recognizing restriction enzymes Sau3a, AluI, BspRI and then analysed using electrophoresis with microdensitometry.
- 2.2. The distribution patterns of restriction DNA fragments proved to be nuclease- and taxon specific.
- 3.3. The observed families of repeated sequences are characterized by different length (from 30 bp to 23 tbp), number of copies in genome (approximately 103 and 106) and supposedly different types of organization and evolutionary age.
- 4.4. The total DNA amount identified in the form of discrete fragments is 16 and 19–21% for magpie and crows, respectively.
- 5.5. The DNA restriction patterns of hybrid forms do not differ from the parental species.
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