Isolation and complete amino acid sequences of eclosion hormones of the silkworm,Bombyx mori |
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| Affiliation: | 1. Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo 113 Japan;2. Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fachu, Tokyo 183, Japan;1. Department of Psychiatry, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8504, St Louis, MO 63110, USA;2. Department of Neurology, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8111, St Louis, MO 63110, USA;3. Department of Radiology, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8111, St Louis, MO 63110, USA;4. Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8111, St Louis, MO 63110, USA;5. Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8504, St Louis, MO 63110, USA;1. Department of Pure and Industrial Chemistry, University of Nigeria, Nsukka, Enugu State, Nigeria;2. Department of Chemistry, Clemson University, Clemson, SC 29634, USA;3. Department of Chemistry, Aydin Adnan Menderes University, 09010 Aydin, Turkey;4. Department of Biology, Aydin Adnan Menderes University, 09010 Aydin, Turkey;5. Department of Chemistry, Zonguldak Bulent Ecevit University, 67100 Zonguldak, Turkey;6. Department of Biophysics, Faculty of Medicine, Aydin Adnan Menderes University, 09010 Aydin, Turkey;7. Department of Mathematics and Science Education, Faculty of Education, Ondokuz Mayıs University, 55139 Samsun, Turkey;8. School of Chemical Sciences, The University of Auckland, New Zealand;9. Department of Chemistry, University of Akron, Akron, OH 44325, USA;1. University Department of Infectious and Tropical Diseases & WHO Collaborating Centre for TB/HIV and TB Elimination, University of Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy;2. Centre for Tropical Diseases, Sacro Cuore Don Calabria Hospital, Via Sempreboni 5, 37024, Negrar, Italy;3. Unità Distretto Territoriale, ASST Spedali Civili di Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy;4. Unit of Hygiene, Epidemiology and Public Health, University of Brescia, Viale Europa 11, 25123, Brescia, Italy;5. Centro Pneumologico Preventivo, ASST Spedali Civili di Brescia, Piazzale Spedali Civili 1, 25123, Brescia, Italy;6. Clinical Epidemiology Unit, Lazzaro Spallanzani National Institute for Infectious Diseases, Via Portuense, 292, 00149, Rome, Italy;7. IRCCS San Raffaele Scientific Institute, Via Olgettina Milano, 60, 20132, Milan, Italy;1. Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, PR China;2. State Key Laboratory of Long-Acting and Targeting Drug Delivery System, Shandong Luye Pharmaceutical Co., Ltd, Yantai 264003, PR China;3. Department of Chemical Engineering and Material Science, Wayne State University, Detroit, MI 48202, USA;1. Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China;2. Tsinghua Fly Center, Tsinghua University, Beijing 100084, China;3. School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China;4. College of Bioengineering, Hubei University of Technology, Wuhan 430068, China;5. Sichuan Academy of Grassland Science, Chengdu 611731, China;6. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA;7. Department of Genetics, Stanford University, Stanford, CA 94305, USA;8. Department of Molecular and Cellular Medicine, College of Medicine, Texas A&M Health Science Center, College Station, TX 77843, USA |
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| Abstract: | Four closely related eclosion hormones (EHs), EH-I, -II, -III and -IV, were purified from 770,000 pharate adult heads of the silkworm, Bombyx mori. The complete amino acid sequence was determined by Edman degradation of peptide fragments generated by enzymatic digestion. Preliminary results have been reported (Kono et al., Agric. biol. Chem.51, 2307–2308, 1987). Accumulated data suggested that EH-IV has the longest sequence consisting of 62 residues. EH-I and -III have N-termini truncated by two residues. EH-I and -II terminate at position 61. The removal of the C-terminal leucine in EH-I and -II might have occurred artificially during extraction. |
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