Methylation patterns in 5' terminal regions of pluripotency-related genes in bovine in vitro fertilized and cloned embryos |
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| Authors: | Jie Lan Song Hua Hailin Zhang Yongli Song Jun Liu Yong Zhang Key Laboratory of Animal Reproductive Physiology & Embryo Technology Institution of biotechnology College of Veterinary Medicine Northwest A & F University Yangling China |
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| Institution: | Jie Lan,Song Hua,Hailin Zhang,Yongli Song,Jun Liu,Yong Zhang Key Laboratory of Animal Reproductive Physiology & Embryo Technology,Institution of biotechnology,College of Veterinary Medicine,Northwest A & F University,Yangling 712100,China |
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| Abstract: | In order to investigate DNA methylation profiles of five pluripotency-related genes(Oct4,Sox2,Nanog,Rex1 and Fgf4)during bovine maternal to zygotic transition(MZT)in both in vitro fertilized(IVF)and nuclear transfer(NT)embryos,sodium bisulfite sequencing method was used to detect DNA methylation levels,accompanied by the statistical analysis of embryo developmental rates.The results showed that Oct4,Nanog,Rex1 and Fgf4 were respectively demethylated by 25.22%(P < 0.01),3.84%(P > 0.05),31.82%(P < 0.01)and 10... |
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| Keywords: | bovine DNA methylation Fgf4 Nanog Oct4 Rex1 Sox2 |
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