Xenobiotic oxidation by cytochrome P-450-enriched extracts of Streptomyces griseus |
| |
Authors: | M K Trower F S Sariaslani F G Kitson |
| |
Institution: | E.I. Du Pont de Nemours & Co. (Inc.), Central Research and Development Department, Wilmington, DE 19880-0228. |
| |
Abstract: | Crude extracts of Streptomyces griseus grown on soybean flour-enriched medium contain high levels of cytochrome P-450. The cytochrome P-450-enriched fractions, obtained by ammonium sulfate fractionation (30-50% saturation), catalyze the NADPH-dependent oxidation of a variety of xenobiotics when complemented with both spinach ferredoxin:NADP+ oxidoreductase and spinach ferredoxin. Reactions observed are aromatic, benzylic and alicyclic hydroxylations, O-dealkylation, non-aromatic double bond epoxidation, N-oxidation and N-acetylation. |
| |
Keywords: | |
|
|