超级杂交水稻 TIR1 类似基因 cDNA 的克隆与生物信息学分析

生长素受体TIR1通过形成SCFTIR1复合体与生长素直接结合, 即为Aux/IAA在26S 蛋白酶体降解过程中的关键蛋白质。在Blas t检索和生物信息学分析的基础上设计特异引物, 以超级杂交水稻(Oryz a sativa)亲本株1S为材料, 通过RT-PCR扩增并经T-A克隆后测序, 获得一条长度为2 219 bp 的序列, 其开放阅读框长度为1 764 bp, 编码含587个氨基酸残基的肽链。该序列经生物信息学分析发现, 其与拟南芥TIR1相似性为77%, 同样具有2个保守的结构域, 即F-box和亮氨酸富集重复区域(LRR),且都不具有跨膜结构域和信号肽。该cDNA序列命名为OsTIR1。

Cloning and Bioinformatics Analysis of TIR1 cDNA from Super Hybrid Rice Parental Line 1S

The auxin receptor TIR1, which can directly bind to auxin through the formation of the SCFTIR1 complex , is the key protein in the Aux /IAA degradation pathway of the 26S proteasome. In this research, we designed a specific primer according to the results of a BLAST search of bioinformatics data; the super hybrid rice parental line 1S was used as the plant material. After RT-PCR and T-A cloning, a 2 219 bp cDNA sequence was obtained through sequencing. Its open reading frame (ORF) was 1 764 bp long and coded for a polypeptide of 587 amino acids. Bioinformatics analysis revealed that the polypeptide shares 77% similarity with AtTIR1. Both polypeptides contain an F-box motif and a leucine-rich repeat domain but no transmembrane helices or signal peptide. The cDNA sequence was designated as OsTIR1 and submitted to GenBank.