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Enzymic alcoholysis of blackcurrant oil
Authors:Miroslav Vacek  Marie Zarevúcka  Zdeněk Wimmer  Karel Stránský  Martina Macková  Kateřina Demnerová
Affiliation:(1) Institute of Chemical Technology, Faculty of Food and Biochemical Technology, Technická 5, CZ-160 28 Prague 6, Czech Republic;(2) Institute of Organic Chemistry and Biochemistry AS CR, Flemingovo nám"ecaron"stí 2, CZ-166 10 Prague 6, Czech Republic
Abstract:Alcoholysis of blackcurrant oil mediated by Pseudomonas fluorescens lipase performed at 30thinsp°C in ethanol (96%, v/v) used both as a solvent and as a reactant. After 16 h, 95% of triacylglycerols present in the oil was converted into a mixture consisting of fatty acid ethyl esters, free fatty acids, monoacylglycerols and diacylglycerols. The highest amount of fatty acid ethyl esters (52%) was achieved after 8 h.
Keywords:alcoholysis  blackcurrant oil  lipase  Pseudomonas fluorescens  triacylglycerol
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