Cytotoxic abietane-derivative diterpenoids of Salvia lachnostachys |
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| Institution: | 1. Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China;2. Department of Gynaecology and Obstetrics, China–Japan Union Hospital, Jilin University, Changchun 130033, PR China;3. School of Life Sciences and Technology, Tongji University, Shanghai 200092, PR China;4. Jilin Provincial Center for Disease Control and Prevention, 130062, PR China;5. Clinical Laboratory, Tumor Hospital of Jilin Province, Changchun 130022, PR China;6. Central Laboratory, The Second Clinical Hospital, Jilin University, Changchun 130041, PR China |
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| Abstract: | A bioassay-guided fractionation of Salvia lachnostachys Benth leaf extract led to the isolation of three known diterpenes, namely fruticuline A (1), fruticuline B (2) and 7,20-dihydrofruticuline A (3), together with two new compounds, 4 and 5. The structures were mainly elucidated by 1D and 2D NMR spectroscopy and HRESIMS. The cytotoxic activity of the crude ethanol extract, the semi-purified fractions (A-E) and compounds 1, 2 and 4 were evaluated against seven human cancer cell lines and the normal cell line HaCat. The ethanol extract showed activity against all tested cell lines (GI50 25.0?44.0 μg/mL). Among the fractions, the greatest activity was exhibited by fraction A (eluted with hexane), which inhibited the growth of all tested cell lines with GI50 of 3.9?19.5 μg/mL. Compounds 1 and 4 were the most active, inhibiting the growth of U251, MCF-7, NCI-ADR/RES, 786.0, NCI-H460, PC-3, OVCAR-03 and HaCat cell lines with GI50 < 10 μM. Compound 2 showed moderate activity against MCF-7, NCI-H460, OVCAR-03, K562 and HaCat, with GI50 varying 19.9?29.3 μM. |
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| Keywords: | Lamiaceae Diterpenes fruticuline A Cytotoxic activity |
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