| 固定化真菌漆酶降解氯苯嘧啶醇农药 |
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| 引用本文： | 尤其,洪宇植,王林,肖亚中,梁睿. 固定化真菌漆酶降解氯苯嘧啶醇农药[J]. 生物学杂志, 2007, 24(1): 44-46 |
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| 作者姓名： | 尤其 洪宇植 王林 肖亚中 梁睿 |
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| 作者单位： | 1. 安徽大学生命科学学院,合肥,230039
2. 安徽农业大学资源与环境学院,合肥,230036 |
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| 基金项目： | 国家自然科学基金;教育部科技重点项目;安徽省优秀青年科技基金;安徽大学"211工程"学术创新团队基金 |
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| 摘 要： | 在葡萄糖培养基中,栓菌420(Trametes sp.420)经6mmol/L邻甲苯胺诱导,漆酶活力达到4535U/L(愈创木酚法)。用弱阴离子交换层析可分离得到纯化漆酶。以壳聚糖为载体,戊二醛为交联剂对漆酶进行固定化,30g壳聚糖与30U漆酶混合,酶活回收率高达69%。在酸性条件下,固定化漆酶对农药氯苯嘧啶醇具有良好的降解作用。
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| 关 键 词： | 真菌漆酶 固定化 农药降解 氯苯嘧啶醇 壳聚糖 |
| 文章编号： | 1008-9632（2007）01-0044-03 |
| 修稿时间： | 2005-12-26 |
Degradation of pesticide fenamirol by immobilized fungal laccase |
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| YOU Qi,HONG Yu-zhi,WANG Lin,XIAO Ya-zhong,LIANG Rui. Degradation of pesticide fenamirol by immobilized fungal laccase[J]. Journal of Biology, 2007, 24(1): 44-46 |
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| Authors: | YOU Qi HONG Yu-zhi WANG Lin XIAO Ya-zhong LIANG Rui |
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| Affiliation: | 1 School of Life Science, Anhui University, Hefei 230039, China; (2 School of Resource and Environment, Anhui Agriculture University, Hefei 230036, China |
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| Abstract: | Trametes sp.420 produced laccase efficiently in glucose medium when induced by 6 mmol/L o-toluidine,with the highest yield of 4535U/L for substrate guaiacol.Pure laccase was obtained by one-step ion-exchange chromatography.Laccase was immobilized onto the glutaradehyde-pretreated supporter chitosan by means of covalent coupling.The recover ratio of enzyme activity reached 69% when 30g of chitosan was mixed with 30U laccase.Furthermore,the immobilized laccase exhibited good ability to degrade pesticide fenarimol at acidic conditions. |
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| Keywords: | chitosan fenarimol fungal laccase immobilization pesticide degradation |
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