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Microbial inactivation using plasma-activated water obtained by gliding electric discharges |
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| Authors: | G Kamgang-Youbi J-M Herry T Meylheuc J-L Brisset M-N Bellon-Fontaine A Doubla M Naïtali |
| Institution: | INRA, AgroParisTech, UMR763 Bioadhésion et Hygiène des Matériaux, Massy, France; AgroParisTech, INRA, UMR763 Bioadhésion et Hygiène des Matériaux, Massy, France; Laboratoire de Microbiologie du Froid UPRES EA 2123, Universitéde Rouen, IUT d'Evreux, Evreux, France; Laboratoire de Chimie minérale, Département de chimie inorganique, Universitéde YaoundéI, Yaoundé, Cameroon |
| Abstract: | Aim: To evaluate the microbial disinfection efficacy of a plasmachemical solution obtained by the activation of water with gliding electric discharges. Methods and Results: Distilled water was activated for 5 min by a nonthermal quenched plasma of the glidarc type operating in humid air and at atmospheric pressure. The plasma-activated water (PAW) was then used to treat planktonic and adherent cells of Staphylococcus epidermidis , Leuconostoc mesenteroides (as models of Gram-positive bacteria), Hafnia alvei (a Gram-negative bacteria) and Saccharomyces cerevisiae (as a yeast model). The treatments were less efficient on adherent cells than on planktonic cells in the case of bacteria, but not of S. cerevisiae . Inactivation was more effective for bacteria than for the yeast. Conclusions: Significant reductions in microbial populations were achieved in all cases, demonstrating the effectiveness of this new approach to treat contaminated media. Significance and Impact of the Study: PAW is a promising solution with potential application to the decontamination of equipment and surfaces. |
| Keywords: | cold plasma glidarc microbial disinfection plasma discharge in liquids sessile cells |