Characterisation of the effect of a simulated hydrocarbon spill on diazotrophs in mangrove sediment mesocosm |
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Authors: | Rodrigo Gouvêa Taketani Henrique Fragoso dos Santos Jan Dirk van Elsas Alexandre Soares Rosado |
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Institution: | 1.Laboratório de Ecologia Microbiana Molecular, Instituto de Microbiologia,Universidade Federal do Rio de Janeiro,Rio de Janeiro,Brazil;2.Microbial Ecology Department, Center for Ecological and Evolutionary Studies,University of Groningen,Groningen,The Netherlands;3.Centro de Energia Nuclear na Agricultura,Universidade de S?o Paulo,Piracicaba,Brazil |
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Abstract: | An analysis of the effect of an oil spill on mangrove sediments was carried out by contamination of mesocosms derived from
two different mangroves, one with a history of contamination and one pristine. The association between N2 fixers and hydrocarbon degradation was assessed using quantitative PCR (qPCR) for the genes rrs and nifH, nifH clone library sequencing and total petroleum hydrocarbon (TPH) quantification using gas chromatography. TPH showed that the
microbial communities of both mangroves were able to degrade the hydrocarbons added; however, whereas the majority of oil
added to the mesocosm derived from the polluted mangrove was degraded in the 75 days of the experiment, there was only partially
degradation in the mesocosm derived from the pristine mangrove. qPCR showed that the addition of oil led to an increase in
rrs gene copy numbers in both mesocosms, having almost no effect on the nifH copy numbers in the pristine mangrove. Sequencing of nifH clones indicated that the changes promoted by the oil in the polluted mangrove were greater than those observed in the pristine
mesocosm. The main effect observed in the polluted mesocosm was the selection of a single phylotype which is probably adapted
to the presence of petroleum. These results, together with previous reports, give hints about the relationship between N2 fixation and hydrocarbon degradation in natural ecosystems. |
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Keywords: | Diazotrophs Mangrove sediment Microbial community Oil degradation |
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