In vitro reinvigoration of mature olive trees (Olea europaea L.) through micrografting |
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Authors: | M Angeles Revilla José Pacheco Abelardo Casares Roberto Rodríguez |
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Institution: | (1) Laboratorio de Fisiología Vegetal, Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, 33005 Oviedo, Spain |
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Abstract: | Summary Portions (1.0–1.5 cm long) of terminal shoots from selected mature treesOlea europaea L. cv. Arbequina, micrografted in one phase ontoin vitro juvenile shoots, resulted in the restoration of shoot-bud proliferation and rooting competence. Although higherin vitro survival rates were obtained after a second repeated micrografting, the reinvigoration ratio of the regenerated shoots, indicated
by proliferation and rooting ability, was not improved after two phases of micrografting. Thus, one-phase micrograft allows
for a successful micropropagation system for olive trees. The cuttings obtained from successive pruning of plants produced
through micrografting and growth in soil showed complete restoration of rooting competence, with rooting percentages similar
to those of juvenile microshoots. |
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Keywords: | mature micrografting micropropagation olive rejuvenation reinvigoration |
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