Sex identification of wolf (Canis lupus) using non-invasive samples |
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| Authors: | Natalia Sastre Olga Francino Gabriel Lampreave Vladimir V Bologov José María López-Martín Armand Sánchez Oscar Ramírez |
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| Institution: | (1) Servei Veterinari de Genètica Molecular, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain;(2) Direcció General del Medi Natural, Departament de Medi Ambient i Habitatge, Generalitat de Catalunya, Dr Roux 80, 08017 Barcelona, Spain;(3) Central Forest State Natural Biosphere Reserve, P/o Zapovednik, Nelidovskij rajon, 172513 Tverskaja Oblast, Russia |
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| Abstract: | We have developed new specific primers for sex determination from forensic samples of wolves (Canis lupus), such as hair, saliva, faecal, tooth and urine samples. In order to improve molecular sexing, we performed a multiplex semi-nested
polymerase chain reaction (PCR) and several replicated amplifications per sample to avoid errors in low quantity DNA samples,
such as allelic dropout and false alleles. The sex of individuals is automatically determined by capillary electrophoresis
with a fluorescently labelled internal sex-specific primer from each pair. Our method yielded sex identification on 100% of
invasive samples and 93% of forensic samples, being one of the highest success rates obtained from wild animals. |
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| Keywords: | Canis lupus Wolf Non-invasive samples Sex-specific primers Sex identification |
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