An atomic force microscopy study of Galleria mellonella apolipophorin III effect on bacteria |
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Authors: | Zdybicka-Barabas Agnieszka Januszanis Barbara Mak Pawel Cytryńska Małgorzata |
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Affiliation: | Department of Invertebrate Immunology, Maria Curie-Sklodowska University, Lublin, Poland. |
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Abstract: | Apolipophorin III (apoLp-III) is an abundant hemolymph protein involved in lipid transport and immune response in insects. As revealed by LIVE/DEAD staining, incubation of Gram-negative and Gram-positive bacteria in the presence of Galleria mellonella apoLp-III led to growth inhibition of selected bacteria. An atomic force microscopy (AFM) study of bacterial cells after apoLp-III treatment showed considerable alterations in the cell surface of Bacillus circulans, Klebsiella pneumoniae and Salmonella typhimurium. Our results clearly demonstrate that apoLp-III disturbed the proper structure of the bacterial cell surface. The alterations were dissimilar to those caused by cationic antimicrobial peptide, cecropin B, suggesting a different mode of action against bacteria. The present results indicate that AFM provides a powerful tool for studying the interactions of apoLp-III with microbial cells. |
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