Suppression of prostaglandin synthesis by analogues of cyclic AMP and forskolin in chondrocyte monolayer cultures |
| |
| Authors: | Charles J. Malemud Teresa M. Mills Robert S. Papay |
| |
| Affiliation: | 1. Department of Medicine Case Western Reserve University School of Medicine, Cleveland, OH 44106, U.S.A.;2. Department of Developmental Genetics and Anatomy, Case Western Reserve University School of Medicine, Cleveland, OH 44106, U.S.A. |
| |
| Abstract: | Recent experimental studies indicated that prostaglandin E2 (PGE2) is the most abundant prostanoid synthesized by rabbit articular chondrocytes. Exogenous PGE2 stimulates cyclic AMP (cAMP) synthesis in these cells. Analogues of cAMP and forskolin have now been shown to suppress the biosynthesis of PGE2 in the presence of serum in a time-dependent manner. The most abundant prostanoid, PGE2 was most markedly affected. PGF2α was unaffected. These results indicated that intracellular accumulation of cAMP in chondrocytes and relative resistance of cAMP to phosphodiesterases control prostanoid synthesis in a negative feedback loop. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
