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Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
Affiliation:1. Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA 94158, USA;2. Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA;3. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA;4. Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02142, USA;5. Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA;6. Department of Biochemistry & Molecular Biology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA;7. Department of Biochemistry & Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA;8. Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA;9. Ultima Genomics, Newark, CA 94560, USA;10. Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA;11. Program for Computational and Systems Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA;12. David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA;13. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
Abstract:
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  • Keywords:genetic screens  cell biology  CRISPR  single-cell RNA sequencing  genotype-phenotype map  Perturb-seq  Integrator complex  chromosomal instability  mitochondrial genome stress response
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