Physicochemical characterization and functional analysis of some snake venom toxin proteins and related non-toxin proteins of other chordates |
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Authors: | Subhamay Panda Goutam Chandra |
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Institution: | 1Division of Biological Sciences, Department of Pharmacy, Gupta College of Technological Sciences, Ashram More, G.T. Road, Asansol-713301, West Bengal, India;2Department of Zoology, The University of Burdwan, Golapbag, Burdwan -713104, West Bengal, India |
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Abstract: | Snake venom contains a diverse array of proteins and polypeptides. Cytotoxins and short neurotoxins are non-enzymatic
polypeptide components of snake venom. The three-dimensional structure of cytotoxin and short neurotoxin resembles a three
finger appearance of three-finger protein super family. Different family members of three-finger protein super family are employed
in diverse biological functions. In this work we analyzed the cytotoxin, short neurotoxin and related non-toxin proteins of other
chordates in terms of functional analysis, amino acid compositional (%) profile, number of amino acids, molecular weight,
theoretical isoelectric point (pI), number of positively charged and negatively charged amino acid residues, instability index and
grand average of hydropathy with the help of different bioinformatical tools. Among all interesting results, profile of amino acid
composition (%) depicts that all sequences contain a conserved cysteine amount but differential amount of different amino acid
residues which have a family specific pattern. Involvement in different biological functions is one of the driving forces which
contribute the vivid amino acid composition profile of these proteins. Different biological system dependent adaptation gives the
birth of enriched bio-molecules. Understanding of physicochemical properties of these proteins will help to generate medicinally
important therapeutic molecules for betterment of human lives. |
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Keywords: | Snake venom Cytotoxin Short neurotoxin Three-finger proteins Bioinformatics Physicochemical characterization |
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