Localization and phenotypic expression of a Tol marker in Citrobacter freundii |
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| Authors: | A Marian J J van Vught J de Graaff A H Stouthamer |
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| Institution: | 1. Laboratory for Medical Microbiology, Free University, Postbox 7161, Amsterdam, The Netherlands
2. Biological Laboratory, Microbiology Department, Free University, Amsterdam, The Netherlands
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| Abstract: | Among colicin-A-tolerant mutants of Citrobacter freundii we characterized some as Tol-2 mutants. The Tol-2 mutation results in insensitivity to bacteriocin S6 and an enhanced sensitivity to deoxycholate (DOC), ethylenediaminetetraacetic acid (EDTA) and to ampicillin. The Tol-2 mutation was mapped close near gal and the gene order pro-tol-gal was established in crosses between C. freundii Hfr tol + donor strains and C. freundii tol - acceptor strains. In these crosses a difference was observed in phenotypic expression of the pleiotropic properties of this tol + gene. Expression of resistance to DOC is substantially slower than the expression of EDTA-resistance. This phenomenon may play a disturbing role in those studies on cell envelope mutants, in which resistance to DOC is used as a selected marker. The differences in expression of DOC-and EDTA-resistance are discussed. |
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