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Double-stranded cDNAs of hop stunt viroid are infectious |
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| Authors: | T Meshi M Ishikawa T Ohno Y Okada T Sano I Ueda E Shikata |
| Abstract: | Restriction fragments composed of only hop stunt viroid (HSV) cDNA were prepared from recombinant clone pHS-P4P, which carries four tandemly repeated HSV cDNAs, and inoculated into cucumber cotyledons. The results showed that double-stranded cDNAs consisting of 1 to 3 units of HSV sequences were infectious. In cucumber plants inoculated with these double-stranded cDNAs, infectious RNA molecules indistinguishable from authentic HSV were propagated. |
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