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Changes in the tissue-specific prevalence of translatable mRNAs in transgenic tobacco shoots containing the T-DNA cytokinin gene
Authors:Johan Memelink  Sacco C de Vries  Rob A Schilperoort  Harry C Hoge
Institution:(1) Present address: Department of Molecular Biology, Agricultural University Wageningen, De Dreijen 11, 6703 BC Wageningen, The Netherlands;(2) Molbas Research Group, Department of Plant Molecular Biology, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
Abstract:Two-dimensional gel electrophoresis of in vitro translation products was used to examine differences between the steady state RNA populations of an untransformed tobacco plant line and a non-rooting tobacco shoot line transformed with a T l -DNA segment from Agrobacterium tumefaciens carrying the cytokinin gene (T-cyt). The analysis comprised about 240 translation products representing the more abundant mRNAs. Approximately 8% of the translation products were found to have significantly different concentrations, due to both increases and decreases, when the shoot parts of the transformed and untransformed lines were compared. Only a few of these differences were specific for the comparison of transformed and untransformed shoots. Most of the differences were also observed when the shoot and root parts of the untransformed line were compared. This implies that the shoot or root prevalence of several mRNA species in normal plants is altered in transgenic T-cyt shoots. The observed changes in the mRNA population of transgenic T-cyt shoots are discussed in relation to the transformed phenotype and previously cloned mRNAs showing similar changes in tissue-specific prevalence.
Keywords:cytokinins  in vitro translation  T-cyt gene  T-DNA  tobacco
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