Purification and properties of proteases produced byalternaria alternata (Fr.) Keissl,regulation of production by fructose |
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Authors: | M Patil N V Shastri |
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Institution: | (1) Department of Biochemistry, Nagpur University, 440010 Nagpur, India |
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Abstract: | A strain ofAlternaria alternata (Fr.) Keissl, when grown on wheat bran Czapek Dox medium was found to secrete one neutral and two alkaline proteases. The
purified enzymes were found to be endo peptidases, the alkaline proteases being serine proteases and neutral proteases being
cysteine proteases. Fructose when added to the culture medium was found to give rise to a new neutral protease at the expense
of the neutral protease produced in the absence of fructose and was also found to enhance the production of alkaline proteases.
It also appears that fructose modifies the alkaline proteases with respect to some characteristics such asV
max, Ea etc. Sodium dodecyl sulphate Polyacrylamide gel electrophoresis indicated a significantly altered protein profile in
fructose supplemented medium. |
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Keywords: | Alternaria proteases fructose |
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