用Ciprofloxacin去除传代细胞株中的支原体污染的研究

在应用细胞培养手段的生物学研究和生物工程产品中，支原体污染仍是一个非常棘手的问题。对Ｖｅｒｏ和ＳＰ２／０－Ａｇ１４等细胞，应用Ｃｉｐｒｏｆｌｏｘａｃｉｎ，１０μｇ／ｍｌ处理１４天，支原体检测全部转阴，经４个月的培养、传代、冻存、复苏，每次支原体检测均保持阴性。对去除了支原体的Ｖｅｒｏ和ＩＳＣ－１１６细胞株，测试了其生长特征和功能，均未见受影响。

STUDY ON THE ELIMINATION OF MYCOPLASMA WITH CIPROFLOXACIN FROM CELL CULTURES

The infection of Mycoplasma in cell cultures remains a bothering problem in the biological research using cell biological technology and in the biotechnological products. Treated with Ci-profloxacin (ion g/ml culture medium) for 14 days, vero.. Sp 2/0-Ag 14 and other cell lines became negative in the tests for mycoplasma These decontaminated cell lines kept negative in the above tests after at least 4 months of cultivation, subcultivation storage in the nitrogen and recovery from the liquid nitrogen The growing characteristics of cell line Vero and the secreting activity of hybridoma cell line LSC-H6 which had been both cultured in the Ciprofloxacin-con-tained medium were also tested while no side-effects were found.