| 加压素片段类似物对C6细胞生长的影响 |
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| 引用本文： | 何淼 缪红华. 加压素片段类似物对C6细胞生长的影响[J]. 细胞生物学杂志, 1995, 17(4): 176-180 |
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| 作者姓名： | 何淼 缪红华 |
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| 作者单位： | 中国科学院上海生物化学研究所，上海生理研究所，上海生理研究所，中国科学院上海生物化学研究所 200031，200031 |
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| 摘 要： | 本文采用显微镜观察和逐个细胞突起长度测量及ＭＴＴ等方法研究了添加肽对无血清培养的Ｃ８细胞生长的影响：在培养早期（９－３６ｈ），１０＾－８ｍｏｌ／Ｌ的ＡＶＰ、ＮＬＰＲ或ＺＮＣ（Ｃ）ＰＲ等都能刺激生长；同浓度的ＯＸＴ或ＺＤＣ（Ｃ）ＰＲ在起始时（９－１７ｈ）无明显影响，但稍后（３６ｈ）显示抑制作用。ＭＴＴ染色法分析的结果指出：神经肽促生长作用主要表现在细胞生长前期（１７ｈ）并且是肽浓度依赖的，ＺＮＣ（Ｃ
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| 关 键 词： | 细胞 C6细胞 生长 加压素 神经肽 |
EFFECT OF ARGININE-VASOPRESSIN SHORT ANALOGS ON THE GROWTH OF C 6 CELLS |
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| HE Miao MIAO Hong-hua CHEN Xiu-fang and DU Yu-Cang. EFFECT OF ARGININE-VASOPRESSIN SHORT ANALOGS ON THE GROWTH OF C 6 CELLS[J]. Chinese Journal of Cell Biology, 1995, 17(4): 176-180 |
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| Authors: | HE Miao MIAO Hong-hua CHEN Xiu-fang and DU Yu-Cang |
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| Affiliation: | HE Miao MIAO Hong-hua CHEN Xiu-fang and DU Yu-CangShanghai Institute of Biochemistry and Shanghai Institutel of Physiology,Chinese Academy ofSciences,Shanghai 200031 |
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| Abstract: | The effects of synthetic peptides on the growth of C6 cells in the serum-free culture were microscopically observed and quantitatively measured, and spectrodensitometrically determined by means of MTT staining. In early stage of incubation; (9-36 h),10-8 mol/L arginine-vaso-pressin (AVP), NLPR or ZNC(C)PR markedly stimulated cell growth while oxytocin (OXT) or ZDC(C)PR showed a significant inhibition at h-36. Results generated from MTT experiments confirmed that the stimulating effect of these peptides mainly appeared in the early stage of cell growth (9-17h) and were concentration-dependent. The lowest effective dose of ZNC (C) PR (10-10mol/L) was hundreds times lower than AVP. When C6 cells were in a conditioning growth situation, both ZNC(C)PR and NLPR (10-8mol/L) still markedly showed a facilitation on the neurites elongation. |
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| Keywords: | arginjne-vasopressin Synthetic analogs C6 cell line Growth |
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