| 脑啡肽对小鼠神经母细胞瘤细胞脂褐素影响的实验研究 |
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| 引用本文： | 孙晓江,孙九伶.脑啡肽对小鼠神经母细胞瘤细胞脂褐素影响的实验研究[J].细胞生物学杂志,1995,17(4):189-192. |
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| 作者姓名： | 孙晓江 孙九伶 |
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| 作者单位： | 上海第二医科大学附属仁济医院神经生物学实验室，上海第二医科大学附属仁济医院神经生物学实验室，上海第二医科大学附属仁济医院神经生物学实验室，上海第二医科大学附属仁济医院神经生物学实验室，上海第二医科大学附属仁济医院神经生物学实验室，上海第二医科大学附属仁济医院神经生物学实验室，承德医学院附属医院神经生物学实验室，承德医学院附属医院神经生物学实验室 上海 200001，上海 200001，上海 200001，上海 200001，上海 200001，上海 200001 |
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| 基金项目： | 上海高等院校科学发展基金，河北省自然科学基金 |
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| 摘 要： | 本文应用小鼠神经母细胞瘤细胞Ａ２（ＮＢ－Ａ２）无血清培养建立的神经细胞老化实验研究模型，以显微荧光分光光度术测定单个细胞内脂褐素荧光值作为细胞老化指标，观察了脑啡肽（ＬＥ）对ＮＢＡ２细胞内脂褐素荧光值的影响。结果表明ＬＥ可显著降低细胞内的脂褐素荧光值（Ｐ〈０．０１）。提示ＬＥ可延缓ＮＢＡ２细胞的老化进程。
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| 关 键 词： | 神经母细胞瘤 细胞 脂褐素 脑非肽 肿瘤 |
AN EXPERIMENTAL ANALYSIS OF THE EFFECT OF LEUCINE ENKEPHALIN ON LIPOFUSCIN OF MOUSE NEUROBLASTOMA A_2 CELLS |
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| Sun Xiaojiang,Cai Yan,Su min,ct al.AN EXPERIMENTAL ANALYSIS OF THE EFFECT OF LEUCINE ENKEPHALIN ON LIPOFUSCIN OF MOUSE NEUROBLASTOMA A_2 CELLS[J].Chinese Journal of Cell Biology,1995,17(4):189-192. |
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| Authors: | Sun Xiaojiang Cai Yan Su min ct al |
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| Abstract: | Serum-free cultures of mouse neuroblastoma A2 (NBA2) cells were used as the experimental model for the study of neuronal aging, with microfluorometry of the autofluorescencc of lipofuscin as the index of neuronal aging. The results showed that fluorescent value (F.V.) of lipofuscin increased progressively with prolonging of culture days. The results implied that NBA2 cells in scrum-free culture change from division and reproduction into differentiation and maturation, reflecting the process of neuronal aging.After addition of Icucinc cnkephalin (LE) separately into the culture medium, the results showed that F. V. of lipofuscin was decreased in 5,10 and 15 day treatment group (P<0.01). The result suggested that LE had the property to retard the accumulation of lipofuscin and produced effect in the direction of delaying NBA2 cells iging. |
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| Keywords: | Leucine enkephalin NBA2 cells lipofuscin Aging |
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