血管紧张素Ⅱ对培养心肌细胞蛋白质和DNA合成的影响

本文利用体外细胞培养技术，观察了血管紧张素Ⅱ对Ｗｉｓｔａｒ乳鼠的心肌细胞和心肌组织中非心肌细胞的促生长作用。结果表明：随ＡｎｇⅡ浓度的升高，心肌细胞直径、总蛋白含量及＾３Ｈ－Ｌｅｕ掺入率均呈剂量依赖性增加，而各组间＾３Ｈ－ＴｄＲ掺入率及心肌细胞数目则无明显变化。相反，ＡｎｇⅡ在引起心肌组织中非心肌细胞（主要是成纤维细胞）的＾３Ｈ－Ｌｅｕ掺入率增加的同时，也引起其＾３Ｈ－ＴｄＲ掺入率及细胞数目的增加

EFFECT OF ANGIOTENSIN I ON PROTEIN AND DNA SYNTHESIS OF CULTURED MYOCARDIAL CELLS

Recently, there are accumulating evidences for the existence of an local tissue renin-angio-tensin system (RAS) in the heart which maybe is associated with the hypertrophic response. In this study, cell culture technique in vitro was used-to investigate the hypertrophic and pro-liferiative effect of angiotensin I on the cultured neonatal rat myocytes and nonmyocytes. The results showed that in myocytes culture, angiotensin I induced increases in cell diameter, protein content and 3H-Leucine incorporation in concentration-dependent manner without changing the rate of DNA synthesis and cell numbers. In contract, angiotensin I caused incerases of DNA synthesis and cell numbers in nonmyocytes cultures. These results indicate that angiotensin 11 can induce the hypertrophy for cardiac myocytes and the proliferation for cardiac nonmyocytes (mostly fibroblasts).