Protective potential of resveratrol against oxidative stress and apoptosis in Batten disease lymphoblast cells |
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Authors: | Yoon Dong-Ho Kwon Oh-Youn Mang Ji Young Jung Myung Jin Kim Do Yeon Park Yoon Kyung Heo Tae-Hwe Kim Sung-Jo |
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Institution: | aDepartment of Biotechnology, Hoseo University, 165, Baebang, Asan, Chungnam 336-795, Republic of Korea;bBokja Girl’s High School, Sunghwang, Chonan, Chungnam 330-130, Republic of Korea;cCollege of Pharmacy, Catholic University of Korea, Bucheon 420-743, Republic of Korea |
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Abstract: | Batten disease (BD) is the most common form of a group of disorders called neuronal ceroid lipofuscinosis, which are caused by a CLN3 gene mutation. A variety of pathogenic lysosomal storage disorder mechanisms have been suggested such as oxidative stress, endoplasmic reticulum (ER) stress, and altered protein trafficking. Resveratrol, a stilbenoid found in red grape skin, is a potent antioxidant chemical. Recent studies have suggested that resveratrol may have a curative effect in many neurodegenerative diseases. Therefore, we investigated the activities of resveratrol at the levels of oxidative and ER stress and apoptosis factors using normal and BD lymphoblast cells. We report that the BD lymphoblast cells contained low-levels of superoxide dismutase-1 (SOD-1) due to the long-term stress of reactive oxygen species. However, when we treated the cells with resveratrol, SOD-1 increased to levels observed in normal cells. Furthermore, we investigated the expression of glucose-regulated protein 78 as an ER stress marker. BD cells underwent ER stress, but resveratrol treatment resolved the ER stress in a dose-dependent manner. We further demonstrated that the levels of apoptosis markers such as apoptosis induce factor, cytochrome c, and cleavage of poly (ADP)-ribose polymerase decreased following resveratrol treatment. Thus, we propose that resveratrol may have beneficial effects in patients with BD. |
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Keywords: | Batten disease Resveratrol Antioxidant Apoptosis |
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