In silicio search for genes encoding peroxisomal proteins in Saccharomyces cerevisiae |
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| Authors: | Arnoud J Kal Ewald H Hettema Marlene van den Berg Marian Groot Koerkamp Lodewijk van Ijlst Ben Distel Henk F Tabak |
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| Institution: | (1) Departments of Biochemistry, University of Amsterdam, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands;(2) Clinical Chemistry, University of Amsterdam, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands;(3) Present address: Imperial Cancer Research Fund, Gene Expression Control Laboratory, 44 Lincoln's Inn Fields, WC2A 3PX London, UK |
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| Abstract: | The biogenesis of peroxisomes involves the synthesis of new proteins that after, completion of translation, are targeted to
the organelle by virtue of peroxisomal targeting signals (PTS). Two types of PTSs have been well characterized for import
of matrix proteins (PTS1 and PTS2). Induction of the genes encoding these matrix proteins takes place in oleate-containing
medium and is mediated via an oleate response element (ORE) present in the region preceding these genes. The authors have
searched the yeast genome for OREs preceding open reading frames (ORFs), and for ORFs that contain either a PTS1 or PTS2.
Of the ORFs containing an ORE, as well as either a PTS1 or a PTS2, many were known to encode bona fide peroxisomal matrix proteins. In addition, candidate genes were identified as encoding putative new peroxisomal proteins.
For one case, subcellular location studies validated the in silicio prediction. This gene encodes a new peroxisomal thioesterase. |
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| Keywords: | Thioesterase Saccharomyces cerevisiae peroxisome oleate-response element (ORE) |
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