In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica |
| |
Authors: | P Srivastava M Singh P Mathur and R Chaturvedi |
| |
Institution: | (1) Department of Biotechnology, Indian Institute of Technology, Guwahati-781039 Assam, India;(2) School of Biotechnology, VIT University, Vellore, 632014, Tamilnadu, India |
| |
Abstract: | A novel method of organogenesis in neem (Azadirachta indica A. Juss.) from unfertilized ovaries is described. The Murashige and Skoog’s (MS) medium with 9 % sucrose, 1 μM 2,4-dichlorophenoxyacetic
acid (2,4-D) and 5 μM 6-benzylaminopurine (BAP) was the best for callus induction from unfertilized ovaries. However, further
proliferation of callus occurred better on MS medium supplemented with 0.5 μM 2,4-D either alone or in combination with 4.5
μM kinetin. Maximum shoot regeneration (78 %) was observed when calli, induced from ovaries of 4 mm size flower buds and proliferating
on MS + 0.5 μM 2,4-D, were subcultured to MS medium containing 5 μM BAP. Histological analysis revealed that 4 mm sized flower
bud corresponds to a 2-nucleate stage of embryo sac. The shoots were then multiplied by forced axillary branching on MS medium
supplemented with 1.0 μM BAP and 250 mg dm−3 casein hydrolysate. The shoots could be rooted on 1/4 strength MS medium supplemented with 0.5 μM indole-3-butyric acid (IBA)
at a frequency of 79 %. Cytological analysis by root tip squash preparations revealed that all the plantlets were diploids.
These plants were subsequently hardened and established in soil with transplantation rate of 81.8 %. |
| |
Keywords: | Additional" target="_blank">Additional adventitious shoot proliferation auxins callus culture casein hydrolysate cytokinins embryo sac histology ovary culture |
本文献已被 SpringerLink 等数据库收录! |
|