Plant cells do not properly recognize animal gene polyadenylation signals |
| |
Authors: | Arthur G Hunt Nathan M Chu Joan T Odell Ferenc Nagy Nam-Hai Chua |
| |
Institution: | (1) Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, 10021 New York, NY, U.S.A.;(2) Present address: Department of Agronomy, University of Kentucky, 40546 Lexington, KY, U.S.A.;(3) Present address: Department of Biology, Yale University, 06511 New Haven, CT, U.S.A.;(4) Present address: DuPont Experimental Station, E402/4243, 19898 Wilmington, DE, U.S.A. |
| |
Abstract: | Summary We have introduced chimeric genes containing polyadenylation signals from a human gene and two animal virus genes into tobacco cells. We see, in all three cases, inefficient and aberrant utilization of the foreign polyadenylation signals. We find that a chimeric gene carrying the polyadenylation site of the human growth hormone gene is polyadenylated at three sites in the vicinity of the site that is polyadenylated in human cells. A chimeric gene containing the polyadenylation site from the adenovirus 5 E1A gene is polyadenylated at a site 11 bases downstream from that reported in animal cells. A gene carrying the polyadenylation site from the SV40 early region is polyadenylated some 80 bases upstream from the site that is polyadenylated in animal cells. In all three cases, related mRNAs ending at flanking authentic plant polyadenylation sites can be detected, indicating that the foreign polyadenylation signals are inefficiently utilized in tobacco cells. |
| |
Keywords: | foreign genes plant transformation polyadenylation |
本文献已被 SpringerLink 等数据库收录! |
|