Cell-Specific Expression of the rolC Gene of the TL-DNA of Ri Plasmid in Transgenic Tobacco Plants |
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Authors: | Sugaya, Sumiko Hayakawa, Katsuyuki Handa, Takashi Uchimiya, Hirofumi |
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Affiliation: | Institute of Biological Sciences, University of Tsukuba Tsukuba, Ibaraki, 305 Japan |
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Abstract: | Transgenic tobacco plants possessing a chimeric gene of thepromoter region of the Ri plasmid ORF12 gene (rolC) and a structuralgene of bacterial ß-glucuronidase were obtained. Histochemicalanalysis showed that the chimeric gene is expressed in phloemcells throughout whole plants. Stable inheritance of such cellspecific expression of ORF12 promoter was also confirmed. 2Present address: Research Institute of Agricultural Resources,Ishikawa Agricultural College, Nonoichi-machi, Ishikawa, 921Japan. (Received September 30, 1988; Accepted March 30, 1989) |
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