Insertion of short hepatitis virus A amino acid sequences into poliovirus antigenic determinants results in viable progeny |
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| Authors: | E D Sverdlov S A Tsarev S V Markova V M Rostapshov T L Azhikina I P Chernov A E Gorbalenya M S Kolesnikova L I Romanova N L Teterina |
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| Institution: | M.M. Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow. |
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| Abstract: | In an infectious poliovirus cDNA construct, the determinant encoding antigenic epitope N-Ag1 (in a loop located between two beta-strands in poly-peptide VP1) was altered by site-directed mutagenesis, to be partially similar with the determinants for presumptive epitopes in polypeptides VP1 or VP3 of hepatitis A virus (HAV). The modified constructs proved to be infectious. However, another construct, in which the same locus encoded a 'nonsense' and a relatively hydrophobic amino acid sequence, exhibited no infectivity. These data showed the feasibility of the insertion of foreign sequences in a specific antigenically active locus of the poliovirus icosahedron, and suggest some limitations with respect to the sequences to be 'transplanted'. |
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