HP30‐2, a mitochondrial PRAT protein for import of signal sequence‐less precursor proteins in Arabidopsis thaliana |
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| Authors: | Claudia Rossig John Gray Oscar Valdes Sachin Rustgi Diter von Wettstein Christiane Reinbothe Steffen Reinbothe |
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| Institution: | 1. Laboratory of Plant Molecular Genetics and Laboratory of Environmental and Systems Biology, Grenoble‐Alpes‐University, Grenoble, France;2. Department of Biological Sciences, University of Toledo, Toledo, OH 43606, USA;3. Department of Plant and Environmental Sciences, Pee Dee Research and Education Center, Clemson University, Florence, SC 29506, USA;4. Department of Crop and Soil Sciences, Washington State University, Pullman WA 99164‐6420, USA |
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| Abstract: | Chloroplasts and mitochondria contain a family of putative preprotein and amino acid transporters designated PRAT. Here,we analyzed the role of two previously characterized PRAT protein family members,encoded by At3g49560(HP30) and At5g24650(HP30-2),in planta using a combination of genetic,cell biological and biochemical approaches. Expression studies and green fluorescent protein tagging identified HP30-2 both in chloroplasts and mitochondria,whereas HP30 was located exclusively in chloroplasts. Biochemical evidence was obtained for an association of mitochondrial HP30-2 with two distinct protein complexes,one containing the inner membrane translocase TIM22 and the other containing an alternative NAD(P)H dehydrogenase subunit(NDC_1)implicated in a respiratory complex 1-like electron transport chain. Through its association with TIM22,HP30-2 is involved in the uptake of carrier proteins and other,hydrophobic membrane proteins lacking cleavable NH2-terminal presequences,whereas HP30-2's interaction with NDC1 may permit controlling mitochondrial biogenesis and activity. |
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