Co-culture ofFrankia alni subsp.pommerii (strain ACN1
AG
) with birch (Betula papyrifera) protoplasts |
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| Authors: | S Laliberté M Lalonde |
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| Institution: | (1) Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géodésie, Université Laval, G1K 7P4 Ste-Foy, Québec, Canada;(2) Present address: Groupe de Recherche en Ecologie Forestière, Université du Québec à Montréal, Département de Sciences Biologiques, Succ. A, CP 8888, H3C 3P8 Montréal, Québec, Canada |
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| Abstract: | The present study was undertaken to set up an experimental system in which barriers to infection of a non-host plant related to the presence of the cell wall, at the level of recognition and/or the necessity of penetrating the cell wall, might be bypassed. Co-cultures betweenFrankia alni subsp.pommerii (strain ACN1
AG
) andBetula papyrifera protoplasts were established. Betula protoplasts remained viable after 2 weeks with no substantial cell wall regeneration. Suppression of the wall barrier was not sufficient to allowFrankia infection under the conditions tested. The non-infectivity ofFrankia on Betula protoplasts may also reflect difficulties inherent to thein vitro environment, which might not permit duplication of infection mechanisms. |
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| Keywords: | Frankia birch protoplasts in vitro co-culture microscopy |
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