Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage. |
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Authors: | S Gillam C R Astell P Jahnke C A Hutchison rd M Smith |
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Affiliation: | S Gillam, C R Astell, P Jahnke, C A Hutchison, 3rd, and M Smith |
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Abstract: | Oligodeoxyribonucleotide mutagenesis has been used to produce a G----A mutation at nucleotide 557 of the phi X174 genome. This changes the ribosome-binding sequence GAGG of gene E to GAAG without affecting the amino acid, glutamine, encoded by the overlapping gene D. The phi X174rb(E)557 mutant does not lyse infected Escherichia coli C and therefore results in the accumulation of a large number intracellular mature phage particles. Thus, the mutation inactivates production of the gene E lytic product, presumably by blocking translation of gene E, without affecting other phage functions. |
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